QX200™ AutoDG™ Droplet Digital™ PCR System

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR™) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

  • Generates droplets for 96 ddPCR reactions in under 45 minutes
  • Compatible with both the QX100™ and QX200™ Droplet Digital™ PCR Systems
  • A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
  • Guides setup and loading with large, color touch-screen interface and LED lighting
  • User-definable plate layout with increments of 8 wells provides run-to-run flexibility
  • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
  • Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validated PrimePCR™ ddPCR™ Assays are now available for mutation and copy number detection.
  • Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
  • Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve
  • Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
  • Environmental monitoring — test a wide variety of environmental samples such as soil and water
  • Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

Specifications

Starting sample size, µl 20
Automated Droplet Generator capacity 1–96 samples/run
Droplets per 20 µl sample 20,000
QX200™ Droplet Reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
Automated Droplet Generator dimensions (W x D x H) Open: 66 x 56 x 89 cm (26 x 22 x 35 in)
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QX200 Droplet Reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11 in)

Required Instruments

The QX200 AutoDG Droplet Digital PCR System requires the use of the C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module and the PX1™ PCR Plate Sealer.

Required Consumables

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

More Information

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:

  • Copy number variation analysis
  • Rare mutation and sequence detection
  • Gene expression
  • Next-generation sequencing library analysis

More information on www.bio-rad.com page.